Description
The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant 17-Hydroxyprogesterone (17-OHP). Standards or samples and HRP-labeled detection antibody specific to Instant 17-Hydroxyprogesterone (17-OHP) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant 17-Hydroxyprogesterone (17-OHP), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Instant 17-Hydroxyprogesterone (17-OHP) in the samples is then determined by comparing the O.D. of the samples to the standard curve